A Human Genomic Library Enriched in Transcriptionally Active Sequences (aDNA Library)
نویسندگان
چکیده
منابع مشابه
Rapid screening of a human genomic library in yeast artificial chromosomes for single-copy sequences.
A yeast artificial chromosome (YAC) library in Saccharomyces cerevisiae consisting of 30,000 clones with an average insert size of 0.1 megabase pair of human DNA has been generated from primary fibroblast DNA. A YAC vector was modified to enable the recovery of both ends of a human DNA insert in plasmids in Escherichia coli and to confer G418 resistance to mammalian cells. A rapid method for ye...
متن کاملIsolation and chromosomal localization of unique DNA sequences from a human genomic library.
Recombinant bacteriophage lambda from a human genomic library were screened to indentify human DNA inserts having only unique sequences. Unique human inserts were found in about 1% of the phage screened. One recombinant phage, P3-2, was studied in detail. It contains a human insert of 14.7 kilobases with four internal EcoRI cleavage sites. A restriction map was constructed for EcoRI and BamHI s...
متن کاملActive Library Resolution in Active Networks
An active network is a computer network in which new protocols can be installed at run-time in any node within the network. For example, the deployment of Internet multicast technology has been slow because service providers have been reluctant to upgrade and reconfigure their routing nodes. Under the active network scheme, users who desire multicast services can have the service automatically ...
متن کاملPreparing a human membrane and secreted protein-enriched cDNA library using PCR primers derived from a genomic database.
We describe here a strategy for preparing a human membrane and secreted protein (MSP)-enriched cDNA library based on human MSP- and non-MSP-encoding cDNA sequences in the databases. The signal peptide parts of the MSP-encoding cDNA sequences, which currently comprise about half of the estimated total number in humans, were analyzed for common patterns. These patterns form a 'minimal' set of pol...
متن کاملThe Role of Library in a School of Architecture, A Study of the Built Environment Library of SBU
The swing of the pendulum between centralization and decentralization of the higher education (HE) system has generally been towards the former in the Iranian context in the past decade. This has prompted some academics, particularly those who find their disciplines left out to react by highlighting intrinsic differences in teaching and studying methods across the HE spectrum. Built Environment...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genome Research
سال: 2000
ISSN: 1088-9051
DOI: 10.1101/gr.10.6.874